Fig. 5

miR-506 suppressed the Wnt/β-catenin signaling pathway in NPC cells by inhibiting LHX2. a the miR-506 expression and mRNA level of LHX2, Wnt1, β-catenin, E-cadherin, Vimentin, and Twist in CNE2 cells following transfection as determined by RT-qPCR; b the protein level of LHX2, Wnt1, β-catenin, E-cadherin, Vimentin, and Twist in CNE2 cells following transfection as measured by Western blot analysis; c the miR-506 expression and mRNA level of LHX2, Wnt1, β-catenin, E-cadherin, Vimentin, and Twist in 5-8F cells following transfection as determined by RT-qPCR; d protein bands and the protein level of LHX2, Wnt1, β-catenin, E-cadherin, Vimentin, and Twist in 5-8F cells following transfection as measured by Western blot analysis; e the binding of LHX2 and IgG with the promoter region of TCF4 in CNE2 and 5-8F cells as determined by ChIP assay; f the binding of LHX2 with the promoter of TCF4 as determined by dual-luciferase reporter gene assay; g and i mRNA level of TCF4 in CNE2 and 5-8F cells following transfection as determined by RT-qPCR; h and j protein bands and the protein level of TCF4 in CNE2 and 5-8F cells following transfection as measured by Western blot analysis; * p < 0.05, vs. the blank and NC groups; NC, negative control; miR-506, microRNA-506; LHX2, LIM Homeobox 2; NPC, nasopharyngeal carcinoma; E-cadherin, epithelial-cadherin; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; TCF4, transcription factor 4; IgG, immunoglobulin G