Fig. 3
From: Elevated expression of Gab1 promotes breast cancer metastasis by dissociating the PAR complex
Gab1 overexpression dissociates the PAR complex. a Endogenous interaction of Par3 with Par6, aPKC and Gab1 is detected in MDA-MB-231 and SK-BR3 cells. b Overexpression of Gab1 inhibits the interaction of Par3 with Par6 and aPKC through an enhanced Gab1-Par3 interaction in both MDA-MB-231 and SK-BR3 cells. c Knockdown Gab1 interrupts the Gab1-Par3 interaction to fasten the interaction of Par3 with Par6 and aPKC in both MDA-MB-231 and SK-BR3 cells. d Re-expression of a fully functional but not a Par1b/Par3 binding domain deletion mutant Gab1 improves cell migration on the background of Gab1 knockdown. Scale bars: 200 μm e Re-expression of a fully functional but not a Par1b/Par3 binding domain deletion mutant Gab1 inhibits the interaction of Par3 with both Par6 and aPKC