Fig. 4
From: Elevated expression of Gab1 promotes breast cancer metastasis by dissociating the PAR complex
Gab1 overexpression promotes EMT in BCa cells. (a, b) Overexpression of Gab1 decreases the expression of E-cad and ZO-1 and increases the expression of N-cad and Vimentin in both MDA-MB-231 (a) and SK-BR3 (b) cells. (c, d) Knockdown of Gab1 increases the expression of E-cad and ZO-1 and decreases the expression of N-cad and Vimentin in both MDA-MB-231 (c) and SK-BR3 (d) cells. e Re-expression of a fully functional but not a Par1b/Par3 binding domain deletion mutant Gab1 restores the expression of Vimentin as well as represses the expression of E-cad and ZO-1 after Gab1 knockdown in MDA-MB-231 cells. Scale bars: 50 μm, Vim: Vimentin