Fig. 5

Upregulated Gab1 enhances BCa metastasis in vivo. a Overexpression of Gab1 increases the number of metastatic nodes in lung tissues. b Histopathological assessment in lung tissues from 231-vec or 231-Gab1OE cells inoculated mice by H&E staining. c Gab1 expression is detected in lung metastatic nodes by co-staining of Gab1 and H-nuclei. d Knockdown of Gab1 decreases the number of metastatic nodes in lung tissues. e Histopathological assessment in lung tissues from 231-con or 231-shGab1 cells inoculated mice by H&E staining. f Downregulated Gab1 expression was identified in lung tissues from 231-shGab1 cells inoculated mice. g Re-expression of a fully functional but not a Par1b/Par3 binding domain deletion mutant Gab1 improves lung metastasis of BCa. h Histopathological assessment in lung tissues from 231-shGab1-vec, 231-shGab1-Gab1MT1, 231-shGab1-Gab1MT2 or 231-shGab1-Gab1WT cells inoculated mice by H&E staining. i Gab1 expression is increased after re-expression of a fully functional but not a Par1b/Par3 binding domain deletion mutant Gab1 upon Gab1 knockdown. Scale Bar: 1 cm for whole tissue scanning and 100 μm for micrograph in (b), (e) and (h), Scale Bar: 100 μm for IF staining in (c), (f) and (i), H-nuclei: human nuclei, L: lung, T: tumor,**: p < 0.01