Fig. 1

miR-29a is preferentially expressed in the most aggressive glioblastoma subclasses and promotes glioblastoma growth: a) Consensus clustering microRNA expression heatmap using 121 highly informative microRNA expression profiles from 197 glioblastomas demonstrating the expression of miR-29a (arrow) in the astrocytic and neural subclasses. The microRNA based classification of the tumor specimens is indicated at the top of the diagram, and the mRNA-based classification is indicated at the bottom. b) Median survival of each of the microRNA-based glioblastoma subclasses. c) BrdU proliferation assay illustrating the effect of miR-29a, miR-29b, miR-29c or a control mimic (100 nM) on proliferation in human U87 glioblastoma cells. Mean ± SEM (*P < 0.026, unpaired t-test). d) MTT cell growth assay illustrating the effect of lentiviral-mediated overexpression of miR-29a or a control sequence on the growth of primary human glioblastoma stem-like cells (*P < 0.0001, unpaired t-test)