Fig. 2

miR-29a downregulates PTEN in glioblastoma cells. a) LN229 glioblastoma cells transduced with a miR-29a lentivirus or a control virus were transplanted subcutaneously into nude mice (n = 8 animals/group), and tumor volume was measured serially. A significant increase in tumor growth was observed in LN229 glioblastoma cells overexpressing miR-29a (Mean ± SEM, *P < 0.05, unpaired t-test). b) Effect of miR-29a on PTEN expression in LN229 glioblastoma cells or in primary glioblastoma stem-like cells transduced with a control or miR-29a lentivirus. c) Western blot illustrating effect of miR-29a on PTEN expression and AKT phosphorylation (ser473) in LN229 glioblastoma cells transduced with a control or miR-29a lentivirus. d) Analysis of PTEN copy number and microRNA expression data for 470 microRNAs. Data obtained from the TCGA portal for 197 glioblastoma specimens. Circled area identifies miR-29a (PCC = 0.219), miR-29c (PCC = 0.260) and miR-29b (PCC = 0.228) as 3 of the top 4 microRNAs most positively correlated with PTEN copy number in glioblastoma. Open arrow indicates location of miR-29a expression profile