Fig. 3

miR-29a increases proliferation and growth in glioblastoma cells lacking PTEN. a) Fluorescence micrographs (left panels) and data quantitation (right panel) from BrdU proliferation assay investigating the effect of lentiviral-mediated miR-29a overexpression on BrdU (red) incorporation into nuclei (DAPI, blue) by human U251 glioblastoma cells that lack functional PTEN. Data shown in right panel are mean ± SEM. *P < 0.0005, unpaired t-test. Scale approximately 100 μm. b) Fluorescence micrographs (left panels) and quantitation (right panel) from BrdU proliferation assay illustrating the effect of a miR-29a inhibitor (100 nM) on proliferation in PTEN-deficient U251 glioblastoma cells. P < 0.05, unpaired t-test. Data shown are mean ± SEM. *P < 0.0005, unpaired t-test. Scale approximately 100 μm. c) MTT cell growth assay illustrating the effect of a specific miR-29a inhibitor (100 nM) on the growth of PTEN-deficient U251 glioblastoma cells. Data shown are mean ± SEM. *P < 0.01, unpaired t-test