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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: MicroRNA-29a activates a multi-component growth and invasion program in glioblastoma

Fig. 4

miR-29a activates AKT and increases β-catenin in PTEN-deficient glioblastoma cells. a) Effect of miR-29a mimic (100 nM) or a scrambled control mimic on AKT and β-catenin expression in PTEN-deficient U251 glioblastoma cells. b) Effect of miR-29a mimic (100 nM) or a control mimic on GSK3β phosphorylation in PTEN-deficient U251 glioblastoma cells. c) Effect of miR-29a on EphB3 expression in U251, U87 or LN229 glioblastoma cells transduced with a control or a miR-29a lentivirus. d) Western blot illustrating effect of EphB3 siRNA on EphB3 expression and AKT phosphorylation in LN229 glioblastoma cells. e) MTT assay illustrating effect of β-catenin overexpression on cell growth in human glioblastoma cells. Data shown are mean ± SEM. *P < 0.05, unpaired t-test. f) MTT assay illustrating effect of the miR-29a mimic (100 nM) on survival of U251 glioblastoma cells after camptothecin exposure (75 nM; mean ± SEM, *P < 0.006, unpaired t-test)

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