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Fig. 7 | Journal of Experimental & Clinical Cancer Research

Fig. 7

From: MicroRNA-29a activates a multi-component growth and invasion program in glioblastoma

Fig. 7

Endogenous miR-29a regulates glioblastoma cell invasion in vivo and correlates with patient survival. a) Effect of miR-29a sponge overexpression on Sox4 and HIC5 expression in human U251 glioblastoma cells. b) BrdU proliferation assay illustrating the effect of a miR-29a sponge on U251 glioblastoma cell proliferation. Mean ± SEM. *P < 0.02, unpaired t-test. c) Matrigel invasion assay illustrating effect of miR-29a sponge on U251 glioblastoma cell invasion. d) Fluorescence micrographs of U251 glioblastoma cells transduced with lentiviruses encoding miR-29a, a miR-29a sponge or a control sequence. Scale approximately 3 μm. e) Fluorescence micrograph of mouse brain section obtained 1 week after transplantation of U251 glioblastoma cells transduced with a control (red) or miR-29a sponge lentivirus. Scale approximately 500 μm. f) Kaplan-Meier survival analyses using microRNA expression profiles (n = 197) or mRNA expression profiles (n = 504) obtained from the TCGA portal for glioblastoma. P values are miR-29a (P = 0.038), SOX4 (P = 0.023), HIC5 (P = 0.027), EphB3 (P = 0.045)

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