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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: Extracellular vesicles secreted by hypoxia pre-challenged mesenchymal stem cells promote non-small cell lung cancer cell growth and mobility as well as macrophage M2 polarization via miR-21-5p delivery

Fig. 1

Extracellular vesicles (EV) secreted by hypoxia pre-challenged mesenchymal stem cells (MSCs) promote A549 and H23 cell proliferation, survival, mobility and EMT in vitro. Cells were pre-treated with EVs secreted by naïve (N-EV) or hypoxia pre-challenged (H-EV) MSCs. a and b, CCK-8 cell proliferations assay evaluating viable cell count at different timepoints. cg, A549 and H23 cell apoptosis under normal, hypoxia challenge (1% O2) or cisplatin challenge (5 μM) after N-EV or H-EV treatment. Hypoxia or cisplatin challenge was performed for 24 h before analysis. hi, trans-well assay evaluating A549 and H23 cells invasion after N-EV or H-EV treatment. A549 or H23 cells treated with PBS vehicle were used as negative control (NC). Bar in I indicates 20 μm. jl, western blot detecting epithelial and mesenchymal marker in A549 and H23 cells after treatment with N-EV or H-EV. Statistical analysis results by Student’s t test was marked by # and that by Dunnett’s test were marked by *. * or #, p < 0.05; ** or ##, p < 0.01; ***, p < 0.001

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