Fig. 5

miR-21-5p delivery by H-EV treatment promote NSCLC cell proliferation, survival and mobility by targeting PTEN, PDCD4 and RECK. a–d, western blot detecting PTEN, PDCD4 and RECK protein expression level in A549 or H23 cells after different treatment as described in Fig. 4. e–k, cell functional assay as described in Fig. 1. A549 or H23 cells with or without PTEN, PDCD4 or RECK gene overexpression (O/E) were tested. Wild type, non-treated A549 or H23 cells were used as negative control (NC). Bar in K indicates 20 μm. Tukey’s test was used for statistical analysis. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001