Fig. 7

H-EV promoting tumor growth in vivo was reduced by miR-21-5p inhibition. a and b, tumor volume growth with different MSC-EV treatment in tumor-bearing mice. c–e, tumor weight at day 30 after A549 or H23 cells transplantation. f–h, RT-qPCR detecting miR-21-5p and pre-miR-21 expression level in xenograft tumor tissue samples. i–k, in situ immunohistochemical (IHC) staining of Ki-67 and CD31 in xenograft tumor paraffin section. Bar indicates 10 μm. l, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) in xenograft tumor cells. m–o western blot detecting murine M1/M2 macrophage marker protein iNOS and Arginase-1 in xenograft tumor. Data in f–o were obtained using xenograft tumor established using A549 cells. Tukey’s test was used for statistical analysis. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001