Fig. 3

Expression of apoptosis-related proteins and cell viability in treated MCF-7 cells. Cytochrome c (Cyto c) and apoptosis-induced factor (AIF) proteins that were released from mitochondria and cleaved caspase-9 protein in the cytosol were analysed after 3-day treatments, as was the nuclear translocation of phosphorylated p53 (p-p53) and AIF (a). Simultaneously, cell viability was evaluated by WST-1 proliferation assay on days (d) 1, 3, 5 and 7 (b). * p < 0.05, difference relative to the control (Ctrl) group. # p < 0.05, difference relative to the Pep-1 group. + p < 0.05, difference between Mito and P-Mito groups