Fig. 6

Expression of proteins involved in mitochondrial dynamics and biogenesis in treated MCF7 cells. After 3-day treatments, the levels of the mitochondrial dynamic-related proteins optic atrophy-1 (OPA1), Mitofusin 2 (MFN2), and dynamin-related protein 1 (Drp-1), as well as mitophagy-related proteins, namely, full-length PTEN-induced putative kinase 1 (PINK-1-L) and Parkin, were analysed and quantified (a). Mitochondrial morphology in terms of MitoTracker Red staining (b) was quantified with an automatic classification system to classify morphology into three distinct morphological subtypes, namely, fragmented, tubular and elongated (b, right panel). Mitochondrial biogenesis was evaluated by analysing the copy number of mitochondrial DNA (mtDNA) relative to that of the β-actin gene (c), and mitochondrial mass was measured with 10-N-nonyl acridine orange (NAO) staining and flow cytometry (d). * p < 0.05, difference relative to the control (Ctrl) group. # p < 0.05, difference relative to the Pep-1 group. + p < 0.05, difference between the Mito and P-Mito groups