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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: PCK1 negatively regulates cell cycle progression and hepatoma cell proliferation via the AMPK/p27Kip1 axis

Fig. 1

Suppression of PCK1 expression promotes cell proliferation by inducing G1/S phase transition in hepatoma cells. a-d Cell proliferation curves and colony formation assay. a, b SK-Hep1 and MHCC-97H cells were infected with adenoviruses expressing PCK1 or GFP control. PCK1 overexpression (PCK1-OE) was confirmed by western blotting. c, d Endogenous PCK1 was knocked out in PRF/PLC/5 cells by CRISPR-Cas9 and confirmed by western blotting. Cells were seeded into 96-well plates at 1 × 103 cells/mL and counted every 24 h in triplicate for cell proliferation analysis. Cells were seeded in 6-well plates at 1 × 103 cells/well and cultured for 2 weeks for colony formation assays. e, f Flow cytometric analysis. e PCK1-OE SK-Hep1 cells and f PCK1-KO cells were collected at different time points and subjected to flow cytometry. Populations of cells at G1 and S phases are indicated as percentages of the whole cell population. Data represent the mean ± SD of three independent experiments; (means ± SD; *P < 0.05, **P < 0.01)

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