Fig. 2

PCK1 controls the expression of cell cycle-related genes. a Heat map representing the differential expression of E2F family members, transcription factors, and E2F target genes as determined by RNA-Seq in PCK1-OE and GFP control cells. Hepatoma cells infected with adenoviruses expressing PCK1 (AdPCK1) or vector control (AdGFP) were subjected to RNA-Seq analysis. b–e Differentially expressed genes (DEGs) were validated in PCK1-OE and PCK1-KO hepatoma cells by RT-qPCR and western blot analysis. PCK1-OE SK-Hep1 cells and PCK1-KO cells were treated as described in Fig. 1. b, c mRNA expression levels of the indicated genes were measured by qRT-PCR. d, e Western blot analysis of the indicated genes in PCK1-OE and PCK1-KO cells. β-actin was used as a loading control. Relative levels of target proteins were measured by densitometry. Data are presented as the mean ± SD of three independent experiments; *P < 0.05, **P < 0.01