Fig. 7
ZNF326 promotes the tumour xenografts formation in nude mice. ZNF326 promotes glioma tumorigenesis. The subcutaneous injection of U87 cells stably expressing ZNF326 (puromycin screening) into nude mice (n = 5) significantly accelerated tumor formation compared with the control group (n = 5) (a, b, c). At the same time, the Ki-67 index (nuclear staining/HPF, CTL vs. ZNF326; 89 ± 9 vs. 178 ± 16, P < 0.05, d) and the downstream target gene expression of Wnt pathway and HDAC7 (e) significantly increased. In contrast, the injection of U251 cells transfected with lenti-shRNA-ZNF326 (puromycin screening) attenuated tumor formation (f-h), Ki-67 index (nuclear staining/HPF, CTL vs. shZNF326; 76 ± 6 vs. 33 ± 4, P < 0.05, i), as well as the Wnt target genes (j). Statistical significance was determined by a two-tailed, unpaired t-test. Columns: mean numbers. Bars: S.D. (*: P < 0.05; **: P < 0.01; ***: P < 0.001)