Fig. 2

Overexpression of PAGE4 in PCa cells. a mRNA expression of PAGE4 in 22RV1, DU145 and PC3 cells after transfected with conditional overexpressing-vector of PAGE4. b Protein expression of PAGE4 in PAGE4-transfected 22RV1 cells with or without treatment of H₂O₂. c d mRNA expression of PAGE4 in PAGE4-transfected DU145 and PC3 cells after H₂O₂ stimulation, *P < 0.05. e DU145 and LNCaP cells were co-transfected PAGE4 with either CLK2 or HIPK1. Cells were treated with H₂O₂ stimulation, and the intracellular ROS were measured, *P < 0.05. All experiments were repeated at least 3 times