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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: DHHC protein family targets different subsets of glioma stem cells in specific niches

Fig. 4

BMI1 constitutes a potential interaction partner in the ZDHHC18/ZDHHC23 protein interaction network in proneural and mesenchymal glioma stem cells (GSCs). a, b Lysates from proneural GSCs (PN12) and mesenchymal GSCs (MES23) were subjected to immunoprecipitation using anti-ZDHHC18 and anti-ZDHHC23 antibodies, respectively, and then (a) immunoblotted with anti-ZDHHC18 and anti-ZDHHC23 antibodies, or (b) the IP-protein complex was subjected to LC/MS-MS analysis. Venn diagrams showing the overlaps between specific peaks of the various IP-protein complexes. c Protein–protein interaction networks indicated by STRING software showing the overlapping peaks in both the proneural and mesenchymal GSCs after immunoprecipitation with anti-ZDHHC18 and anti-ZDHHC23 antibodies, respectively. The shared regions mostly converged on 31 protein targets, generally associated with retrograde transport, nuclear-transcribed mRNA catabolic process, cellular lipid, and glucose metabolic processes, H2A monoubiquitination, hypoxia microenvironment, and glioma development

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