Fig. 5

KLF4 upregulated ITGB4 expression. a-c KLF4 was overexpressed in LN229 cells. Gene expression profiles were obtained by RNA sequencing analysis. The mRNA levels of ITGB4 are listed. d-g KLF4 was overexpressed in LN229 and U251 cells. The expression levels of ITGB4 and KLF4 were detected by western blotting and q-RT-PCR assays. Data represent the mean ± SD of three independent experiments. *** p < 0.001 vs. control. h-k KLF4 was knocked down in LN229 and U251 cells. The expression levels of ITGB4 and KLF4 were detected by western blotting and q-RT-PCR assays. Data represent the mean ± SD of three independent experiments. *** p < 0.001 vs. control