Fig. 7

ITGB4 enhanced KLF4 stability. a-b ITGB4 was knocked down in LN229 and U251 cells. The protein levels of ITGB4 and KLF4 were analysed by western blotting assay. c LN229 and U251 cells with or without ITGB4 knockdown were treated with MG132 or not. The protein levels of ITGB4 and KLF4 were analysed by western blotting assay. d-g LN229 cells with or without ITGB4 knockdown or overexpression were treated with CHX (10 mg/ml) for the indicated times. The half-life of KLF4 was measured. Data represent the mean ± SD of three independent experiments. ** p < 0.01, *** p < 0.001 vs. control. h-i LN229 cells with or without ITGB4 knockdown or overexpression, were transfected with the indicated constructs and the cells then were treated with MG132 for 8 h before collection. The whole-cell lysate was subjected to immunoprecipitation with anti-KLF4 antibodies and western blotted with anti-Ub antibodies to detect ubiquitylated KLF4. j LN229 and U251 cell lysates were subjected to immunoprecipitation with control IgG or anti-KLF4 antibodies. The immunoprecipitates were then detected using the indicated antibodies. k-l ITGB4 was knocked down or overexpressed in LN229 cells. The cell lysates were subject to immunoprecipitation with control IgG or anti-KLF4 antibodies. The immunoprecipitates were then detected using the indicated antibodies