Skip to main content
Fig. 8 | Journal of Experimental & Clinical Cancer Research

Fig. 8

From: Reciprocal regulation of integrin β4 and KLF4 promotes gliomagenesis through maintaining cancer stem cell traits

Fig. 8

KLF4 and ITGB4 played an essential role in glioma tumorigenesis. a-d ITGB4 was knocked down in LN229 and U251 cells with or without overexpressing KLF4. The expression levels of ITGB4, Oct4, Nanog, and KLF4 were analysed by western blotting and q-RT-PCR assays. Data represent the mean ± SD of three independent experiments. *** p < 0.001 vs. control. e-h The CD133-positive populations and mammosphere-forming abilities were analysed. Data represent the mean ± SD of three independent experiments. ** p < 0.01 vs. control. i-l Cell migration and proliferation were analysed by transwell and colony formation assays. Data represent the mean ± SD of three independent experiments. *** p < 0.001 vs. control. m-n The cells were subcutaneously injected into nude mice (n = 6 in each group) for tumour formation. Representative bright-field imaging of the tumours in the mice implanted with the indicated cells. After 5 weeks, mice receiving transplants of the indicated cells were sacrificed. The tumour volume and weight were calculated. ** p < 0.01, *** p < 0.001 vs. control

Back to article page