Fig. 1

NUSAP1 is upregulated in cervical cancer. a NUSAP1 mRNA expression levels were significantly upregulated in cervical cancer tissues compared with normal tissues, as indicated by The Cancer Genome Atlas (COAD) cervical cancer dataset and public GEO cervical cancer datasets GSE7803 and GSE9750. d, e Real-time PCR analysis of NUSAP1 mRNA in ECT1/E6E7 human immortalized cervical epithelial cells and in eight cultured cervical cancer cell lines (d) and seven fresh primary cervical cancer tissues as compared with paired normal tissues (e). f Real-time PCR analysis of NUSAP1 mRNA in six cervical cancer samples without metastasis and eight cervical cancer samples with metastasis within 5 years; GAPDH was used as a loading control. g, h Western blotting analysis of NUSAP1 expression in ECT1/E6E7 human immortalized cervical epithelial cells and eight cultured cervical cancer cell lines (g), and in seven paired fresh primary cervical cancer tissues and adjacent non-cancerous tissue sections (h). i Western blotting results of NUSAP1 expression in six cervical cancer cases without metastasis and eight cervical cancer specimens with metastasis within 5 years; α-tubulin was used as a loading control. All experiments were performed in triplicate. Results are the mean ± SD. **p < 0.01; ***p < 0.001