Fig. 5

NUSAP1 enhances CSCs properties. a, b GSEA of TCGA database showed significant enrichment of stemness gene modules (WONG_EMBRYONIC_STEM_CELL,IVANOVA_HEMOTOPOIESIS_STEM_CELL), in samples with high expression of NUSAP1. NES, normalized enrichment score. c, d Real-time PCR analysis of mRNA expression of multiple pluripotency-associated factors, including Sox2, NONOG, OCT4 and BMI1 in the indicated cells. e Representative images of spheres formed by the indicated cells. Scale bar = 100 μm. Histograms showing the mean numbers of spheres are on the right-hand side. f Hoechst 33342 dye exclusion assay showing that overexpression of NUSAP1 increased, whereas silencing of NUSAP1 decreased, the proportion of SP cells among the indicated cells. Histograms showing the mean percentages of side population cells are on the right-hand side. All experiments were performed in triplicate. Results are the mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001