Fig. 7
Canonical Wnt/β-catenin signaling mediates the effects of NUSAP1 and clinical relevance of NUSAP1-induced β-catenin activation in cervical cancer. a Western blotting analysis confirming the depletion of β-catenin in NUSAP1-transduced cells using a β-catenin-siRNA or XAV-939. b TOP/FOP luciferase activity in the indicated cells (c) Quantification of the invading cells in a matrigel-coated transwell assay. d, e Real-time PCR analysis of EMT markers such as E-cadherin (d) and vimentin (e) in the indicated cells. f Histograms showing the mean number of spheres formed by the indicated cells. g, h. Western blotting analysis of NUSAP1 and nuclear β-catenin protein levels in ten fresh cervical cancer tissues. GADPH and p84 were used as loading controls for the cytoplasmic and nuclear fractions, respectively. Right panel, correlation analyses between the protein expression of NUSAP and nuclear β-catenin in ten fresh cervical cancer samples. i, j. Immunohistochemical analysis of NUSAP1 and nuclear β-catenin protein levels in cervical cancer tissues. Left panel, images of two representative cases. Right panel, percentages of cervical cancer specimens with low or high expression of NUSAP1 relative to nuclear β-catenin expression levels. Results are the mean ± SD. **p < 0.01; ***p < 0.001