Fig. 5
From: miR-190 enhances endocrine therapy sensitivity by regulating SOX9 expression in breast cancer
miR-190 inhibits Wnt/β-catenin signaling by regulating of SOX9. a, Luciferase reporter analysis of Wnt/β-catenin signaling in MDA-MB-231 cells transfected with miR-190 mimics and SOX9 plasmid, as well as in control cells. b, The expression of β-catenin in cells as in (a) was determined by western blotting. C, Localization of β-catenin in cells as in (a) was determined by immunofluorescence staining. d and e, The mRNA and protein expression of downstream targets of Wnt/β-catenin signaling in cells as in (a) was determined by RT-qPCR (d) and western blotting (e). *P < 0.05