Fig. 1

Differential FFAR4 expression in normal and cancerous human breast tissues. Representative examples of IHC staining and H-scores for FFAR4 in human breast tissues. Normal breast tissue: negative staining in normal breast epithelial cells. P1: Patient 1, H-score 40 = 1 x (40% cells staining weakly [1+]) + 2 x (0% cells staining moderately [2+]) + 3 x (0% cells staining strongly [3+]). P2: Patient 2, H-score 200 = 0 x (0% cells staining weakly [1+]) + 2 x (100% cells staining moderately [2+]) + 3 x (0% cells staining strongly [3+]). P3: Patient 3, H-score 300 = 1 x (0% cells staining weakly [1+]) + 2 x (0% cells staining moderately [2+]) + 3 x (100% cells staining strongly [3+]). Scale bar, 200 μm (top panel) and 50 μm (bottom panel)