Fig. 2

Confirmation of the circular structure of circSETD3. a Schematic illustration showed that circSETD3 is located at chromosome 14q32.2 and cyclized from exons 2–6 of SETD3, the PCR products of circSETD3 were confirmed by Sanger sequencing. b The existence of cricSETD3 was validated in HCC and paired non-tumorous tissues as well as Hep3B cells. Divergent primers detected circular RNAs in cDNA but not in gDNA. GAPDH was used as negative control. c PCR for detecting circSETD3 and SETD3 linear form RNA in HCC and paired non-tumorous tissues as well as HepG2 cells treated with or without RNase R digestion, circSETD3 was resistant to RNase R treatment. SETD3, SET domain-containing 3; cDNA, complementary DNA; gDNA, genomic DNA. PCR, polymerase chain reaction. ►◄, convergent primer; ◄►, divergent primer