Fig. 3

CircSETD3 inhibits the proliferation of HCC cells in vitro. a The expression levels of circSETD3 in multiple HCC cell lines. b The expression level of circSETD3 in Huh7 cells were successfully overexpressed by circSETD3 letivirus. c The expression level of circSETD3 in Hep3B cells were knocked down by circSETD3 siRNA. d-g CCK-8 (d and e), colony formation (f), and EdU (g) assays showed the overexpression of circSETD3 inhibited the growth of Huh7 cells while knockdown of circSETD3 promoted the growth of Hep3B cells. h Cell cycle was analysed using flow cytometry after transfection with circSETD3 letivirus or siRNA. Overexpression of circSETD3 induced G1/S arrest in Huh7 cells. Knockdown of circSETD3 relieved the G1/S arrest in Hep3B cells. HCC, hepatocellular carcinoma; NC, negative control. **P < 0.01, ***P < 0.001. Error bars indicate SD