Fig. 3
From: PRDM16 functions as a suppressor of lung adenocarcinoma metastasis
Effect of PRDM16 expression on key EMT markers and cell cycle regulators of lung adenocarcinoma cells in vitro. a and b, Immunoblotting analysis of expression of key EMT-related markers in H1299, Calu-1 and A549 cells treated with indicated plasmids. GAPDH was used as an internal control. The relative protein level of control group was used to normalize protein levels in other groups. c, RT-qPCR analysis of mRNA levels of indicated key EMT-related genes in EV and PRDM16 cells normalized to GAPDH. d, Immunofluorescence staining of indicated key EMT-related markers in EV and PRDM16 cells. e, Immunoblotting analysis of expression of indicated key cell cycle regulators in H1299 and A549 cells treated with indicated plasmids. f, RT-qPCR analysis of mRNA levels of indicated key cell cycle regulators in EV and PRDM16 cells normalized to GAPDH