Fig. 5

PRDM16 functions as a MUC4 transcriptional repressor. a, qPCR analysis of validation PRDM16 target genes from the ChIP-Seq experiment in H1299 cells transfected with EV or PRDM16. b, Transcription level of MUC4 in lung adenocarcinoma compared with normal lung tissues. Data were obtained from the UALCAN database [37]. c, Immunoblotting analysis of MUC4 expression in eight randomly chosen lung adenocarcinoma tissues (C) and matched normal lung tissues (N). d, Immunohistochemistry staining of MUC4 (I) and PRDM16 (II) in the same human lung adenocarcinoma tissue, and MUC4 staining in tumor tissues of mice subcutaneously injected with H1299-EV (III) and H1299-PRDM16 (IV) (magnification: × 200). e, Kaplan-Meier plots of overall survival of patients with lung adenocarcinoma stratified by MUC4 expression. Data were obtained from the Kaplan-Meier plotter database [36]. f and g, Immunoblotting analysis of MUC4 in H1299 and Calu-1 cells treated with indicated plasmids. h and i, Dual-luciferase assays for the effect of PRDM16 on MUC4 transcriptional repression in H1299 and Calu-1 cells