Fig. 7
From: PRDM16 functions as a suppressor of lung adenocarcinoma metastasis
PRDM16-mediated transcriptional repression of MUC4. Deleting the PR domain of PRDM16 increases transcriptional repression of MUC4 by regulating histone modifications on the MUC4 promoter. a, Schematic illustration of ten regions in the MUC4 promoter. b, ChIP-qPCR assays for binding regions of PRDM16 on MUC4 promoter. c, Domain locations within the structure of PRDM16. d, Dual-luciferase assays for H1299 cells transfected with indicated plasmids. e, Immunoblotting analysis of indicated protein levels H1299 cells transfected with EV, PRDM16, PRDM16-ΔPRD. f, ChIP-qPCR analysis of the enrichment of indicated H3 modifications on Region 1 (left) and Region 4 (right) of the MUC4 promoter in H1299 cells transfected indicated plasmids. g, A proposed model to illustrate the role of PRDM16 in EMT. Overexpression of PRDM16 inhibits the EMT process by suppressing the transcription of its target gene MUC4. PRDM16-ΔPRD increases the transcriptional repression of MUC4 by reducing enrichment of H3K9me1 and H3k9ac on its promoter