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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: FAT4 regulates the EMT and autophagy in colorectal cancer cells in part via the PI3K-AKT signaling axis

Fig. 1

FAT4 expression in CRC tissues is weak and can inhibit cell proliferation in vitro. a Immunohistochemical staining of FAT4 in CRC and normal tissues. FAT4 expression was weaker in CRC tissues than in nonmalignant tissues. b FAT4 protein expression in representative samples of CRC tissues and paired adjacent noncancerous tissues. In CRC tissues, the expression level of FAT4 was downregulated. c The FAT4 mRNA expression levels in 100 CRC tissues and paired noncancerous tissues were assessed by qRT-PCR, which showed that FAT4 mRNA expression was significantly decreased in CRC tissues, as demonstrated using the 2-ΔΔct method. *P < 0.05, as determined by the Mann-Whitney U-test. d Levels of FAT4 protein expression in three different CRC cell lines. HCT116 and LOVO cells showed low FAT4 expression, and strong FAT4 expression was detected in SW480 cells. e The MTT assay was used to assess the proliferation of the three CRC cell lines. During the 7-day experimental period, the HCT116 and LOVO cells in which FAT4 was overexpressed showed decreased proliferation compared with the control cells, whereas the SW480 cells transfected with shRNA FAT4 showed increased cell proliferation. *P < 0.05, as determined by Student’s t-test

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