Fig. 2

High expression levels of FAT4 could inhibit the EMT and induce autophagy in CRC. a-c Immunofluorescence staining was performed to detect the expression of FAT4 in cells transduced with FAT4 shRNA or a FAT4-overexpression vector compared with that in cells transfected with the negative control. The results revealed that LC3 expression was elevated in the FAT4-overexpressing LOVO and HCT116 cells, whereas FAT4 knockdown inhibited the expression of LC3 in SW480 cells. a-c In addition, in HCT116 and LOVO cells, FAT4 overexpression increased E-cadherin expression, thereby inhibiting the EMT, whereas in SW480 cells, FAT4 knockdown suppressed E-cadherin expression