Fig. 3

FAT4 can regulate the EMT and autophagy in CRC. FAT4 modulates the expression of EMT and autophagy markers. a-c We performed a western blotting assessment of FAT4, β-catenin, E-cadherin, N-cadherin, vimentin, C-Myc, Twist1, ULK1, LC3 and P62 protein expression in three cell lines with modified FAT4 expression. In FAT4-overexpressing HCT116 and LOVO cells, the expression levels of N-cadherin, vimentin, P62, C-Myc, β-catenin and Twist1 were decreased, whereas E-cadherin and ULK1 expression was enhanced. The LC3-II/LC3-I ratio was higher in the FAT4-overexpressing HCT116 and LOVO cells. SW480 cells transfected with shRNA FAT4 exhibited the opposite outcomes. *P < 0.05, as determined by Student’s t-test