Fig. 5

FAT4 induces autophagy and inhibits the EMT through the PI3K-AKT signaling pathway. a-c The levels of relevant signaling pathway proteins in cells transduced with FAT4 shRNA or a FAT4-overexpression vector were examined by western blotting, and the images were compared with those of NC cells. As shown, FAT4 overexpression visibly decreased the expression levels of PI3K, p-AKT, mTOR, p-p70S6K and p-GSK-3β and induced GSK-3β. In SW480 cells, the knockdown of FAT4 increased the expression of PI3K, p-AKT, mTOR, p-p70S6K and p-GSK-3β and reduced GSK-3β expression compared with the levels observed in the control cells. β-Actin was used as the internal control. *P < 0.05, as determined by Student’s t-test