Fig. 1

HAT1 is upregulated in PDAC and associated with poor prognosis in PDAC patients. a, The GEPIA database revealed that HAT1 expression was significantly upregulated in pancreatic cancer tissues. The boxplot analysis show log2 (TPM + 1) on a log-scale. b, Images of the IHC analyses of HAT1 using TMA (n = 25 normal pancreatic specimens, n = 41 PDAC) tissue sections. Scale bars are shown as indicated. c, Box plots of HAT1 expression as determined by the IHC score using TMA (n = 25 normal pancreatic specimens, n = 41 PDAC) tissue sections. ***, P < 0.001. d and e, for d, Expression of HAT1 as determined by Western blot analysis, in eight paired primary pancreatic cancer tissues (T) and the matched adjacent nontumor tissues (N) from the same patient. β -Tubulin served as a loading control; for e, HAT1 proteins were quantified by ImageJ software. The P values are also shown. f and g, The disease-free and (f) overall survival (g) of the patients with PDAC were computed with the GEPIA web tool. h, The overall survival of the patients with PDAC was computed with the Human Protein Atlas