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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Overexpressed histone acetyltransferase 1 regulates cancer immunity by increasing programmed death-ligand 1 expression in pancreatic cancer

Fig. 2

HAT1 promotes cell proliferation in pancreatic cancer in vivo and in vitro. a-c, PANC-1, MIA PaCa-2 and BxPC-3 cells were infected with lentivirus vectors expressing control or HAT1-specific shRNAs. Forty-eight hours postinfection, the cells were harvested for RT-qPCR analysis (a), MTS assay (b) and colony formation assay (c). The data shown are the mean values ± SD from three replicates. **, P < 0.01; ***, P < 0.001. d-f, PANC-1 cells were infected with control or HAT1-specific shRNAs. Then, 72 h postinfection, the cells were injected subcutaneously into the right dorsal flank of nude mice. After 24 days, the tumors were harvested, photographed (d) and measured (e and f). The data are presented as the mean ± SD (n = 7). ***, P < 0.001. g and h, IHC analysis of the Ki-67 expression in xenografts was performed, and the staining was quantified. All data shown are mean ± SD (error bar) from five replicates. *, P < 0.05

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