Fig. 5

SPRY2 and the expression of markers of EMT and the Wnt/β-catenin pathway. qRT-PCR was conducted to detect the mRNA expression of SPRY2, β-catenin, TCF4, E-cad and VIM in PANC-1 (a) and SW1990 (b) cells. The protein expression of SPRY2, β-catenin, TCF4, E-cad and VIM were detected by western blot analyses in PANC-1 (c) and SW1990 (d) cells. *P < 0.05, **P < 0.01, ***P < 0.001