Table 1 The sequences of primers for qRT-PCR
Gene | Primer sequence |
|---|---|
FOXO3a | |
Forward | 5′-CGGACAAACGGCTCACTCT-3′ |
Reverse | 5′-GGACCCGCATGAATCGACTAT-3′ |
SPRY2 | |
Forward | 5′-CTCGGCCCAGAACGTGATT-3′ |
Reverse | 5′-GGCAAAAAGAGGGACATGACAC-3′ |
β-catenin | |
Forward | 5′-CATCTACACAGTTTGATGCTGCT-3′ |
Reverse | 5′-GCAGTTTTGTCAGTTCAGGGA-3′ |
TCF4 | |
Forward | 5′-AGAAACGAATCAAAACAGCTCCT-3′ |
Reverse | 5′-CGGGATTTGTCTCGGAAACTT-3′ |
E-cad | |
Forward | 5′-CGAGAGCTACACGTTCACGG-3′ |
Reverse | 5′-GGGTGTCGAGGGAAAAATAGG-3′ |
VIM | |
Forward | 5′-GACGCCATCAACACCGAGTT-3′ |
Reverse | 5′-CTTTGTCGTTGGTTAGCTGGT-3′ |
β-actin | |
Forward | 5′-ATCACCATTGGCAATGAGCG-3′ |
Reverse | 5′-TTGAAGGTAGTTTCGTGGAT-3′ |