Fig. 4

Inhibiting autophagy impairs degradation of lamin A/C. MDA-MB-231 and MCF7 cells stably expressing control shRNA (sh-Control) or ATG7-shRNA (sh-ATG7) were treated without or with DOX (10 μM) for 24 h. (a) The expression of ATG7 in MDA-MB-231 and MCF7 cells. (b) Nuclear extracts were prepared and subjected to western blot analysis using antibodies against LC3B, SQSTM1, and lamin A/C. Data was presented as mean ± S.D. (**P < 0.01). (c) The localization of mRFP-LC3 (red), mGFP-LAMP1 (green) and leaked DNA (blue) was evaluated by confocal microscopy. Scale bars: 10 μm