Fig. 1
Involvement of AFAP1-AS1 in the development and progression of PC. a, b and c, the thermal map of PC-related GSE16515, GSE32676 and GSE22780 chips, respectively; d and e, the positive expression of AFAP1-AS1 in cancer tissues and adjacent normal tissues measured by In situ hybridization; f, statistical chart of AFAP1-AS1 positive expression; g and h, RT-qPCR analysis of AFAP1-AS1 expression in PC tissues and cell lines; i, the SP cells with CSC-like properties sorted by flow cytometry; j, RT-qPCR analysis of the expression of CSC markers Oct4, ABCG2, Nestin, CK19 and CD133 as well as AFAP1-AS1 and ACVR1. *, p < 0.05 vs. the PC tissues (n = 75); &, p < 0.05 vs. the HPC-Y5 cell line; #, p < 0.05 vs. the PANC-1 cell line; ^, p < 0.05 vs. the SP cells. The measurement data were expressed as mean ± standard derivation; paired t-test (in panel G) or t-test (in panel j) was performed for comparison between two groups; one-way ANOVA was applied for comparison among multiple groups. The data in panel f were enumeration data, and analyzed by Chi-square test. The experiment was repeated three times. AFAP1-AS1, actin filament-associated protein 1 antisense RNA 1; PC, pancreatic cancer; RT-qPCR, reverse transcription quantitative polymerase chain reaction; SP, side population; CSC, cancer stem cell; ACVR1, activin receptor A type I; ABCG2, ATP-binding cassette subfamily G member 2