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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Cross-talk among AFAP1-AS1, ACVR1 and microRNA-384 regulates the stemness of pancreatic cancer cells and tumorigenicity in nude mice

Fig. 2

Effect of inhibition of AFAP1-AS1 on PANC-1 cell stemness. a, RT-qPCR analysis of AFAP1-AS1expression; b, western blot analysis of CSC makers; c, statistical chart of protein expression; d, sphere formation of PANC-1 cells (× 200); e and f, statistical chart of sphere diameter and spheres per 100 cells of PANC-1 cells determined by sphere formation assay; g, statistical chart of monoclonal formation rate evaluated by clone formation assay; h, proliferation of PANC-1 cells tested by EdU staining (× 200); i, statistical chart of cell proliferation; j, the number of invasive cells determined by Transwell assay (× 200); k, statistical chart of cell invasion; *, p < 0.05 vs. the shRNA-NC group. #, p < 0.05 vs. the empty vector group. All the above data was measurement data and expressed as mean ± standard derivation. One-way ANOVA was applied for comparison among three groups. The t-test was conducted for the comparison between two groups. The experiment was repeated three times. AFAP1-AS1, actin filament-associated protein 1 antisense RNA 1; PC, pancreatic cancer; RT-qPCR, reverse transcription quantitative polymerase chain reaction; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; ACVR1, activin receptor A type I; ABCG2, ATP-binding cassette subfamily G member 2

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