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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Cross-talk among AFAP1-AS1, ACVR1 and microRNA-384 regulates the stemness of pancreatic cancer cells and tumorigenicity in nude mice

Fig. 3

AFAP1-AS1 is involved in regulation of ACVR1 through competitively binding to miR-384. a, AFAP1-AS1 targeted to inhibit miR-384 as verified by dual-luciferase reporter gene assay; *, p < 0.05 vs. the NC group; b, miR-384 targeted to inhibit ACVR1 as confirmed by dual-luciferase reporter gene assay; *, p < 0.05 vs. the NC group; c, heat map of PC-related GSE71989 chip; d, localization of AFAP1-AS1 in cells detected by FISH; e, the binding ability of AFAP1-AS1 to AGO2 evaluated by RIP assay; *, p < 0.05 vs. the IgG group. f, the binding ability of AFAP1-AS1 to miR-384 determined by RNA pull down. All the above data was measurement data and expressed as mean ± standard derivation. The t-test was performed for comparison between two groups, and one-way ANOVA was applied for comparison among multiple groups. The experiment was repeated three times. AFAP1-AS1, actin filament-associated protein 1 antisense RNA 1; NC, negative control; PC, pancreatic cancer; ACVR1, activin receptor A type I; miR-384, microRNA-384; FISH, fluorescence in situ hybridization; RIP, RNA binding protein immunoprecipitation; AGO2, Argonaute 2

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