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Table 1 Primer sequences for RT-qPCR

From: Cross-talk among AFAP1-AS1, ACVR1 and microRNA-384 regulates the stemness of pancreatic cancer cells and tumorigenicity in nude mice

Gene Sequences
AFAP1-AS1 F: 5′-ACTGAAGAGGAACCAGGGACAG-3′
  R: 5′-GGGGAAACTGAAATGAATGAAG-3′
miR-384 F: 5′-TGTTAAATCAGGAATTTTAA-3′
  R: 5′-TGTTACAGGCATTATGA-3′
ACVR1 F: 5′-GTGAAGGTCTCTCCTGCGGTA − 3′
  R: 5′-GCCATCGTTGATGCTCAGTGA − 3′
Oct4 F: 5’-CAAAGCAGAAACCCTCGTGC-3’
  R: 5’-AACCACACTCGGACCACTCG-3’
Nestin F: 5’-ATCCCGTCAGCTGGAAAAGG-3’
  R: 5’-GGTGAGCTTGGGCACAAAAG-3’
CK19 F: 5’-ACCATTGAGAACGCCAGGATT-3’
  R: 5’-TCCAGCACCCAAACACTCAA-3’
CD133 F: 5′-TATAAAGCTTACCATGGCCCTCGTACTCGGCTC -3′
  R:5′-TATAGGATCCTCAATGTTGTGATGGGCTTGTC-3′
ABCG2 F: 5′-CAGGTGGAGGCAAATCTTCGT-3′
  R: 5′-CTTGTACTCCGTCAGCGTGA-3′
GAPDH F: 5’-ACAGTCCATGCCATCACTG-3’
  R: 5’-AGTAGAGGCAGGGATGATG-3’
U6 F: 5’-TGCGGGTGCTCGCTTCGGCAGC-3′
  R: 5′-CCAGTGCAGGGTCCGAGGT-3′
  1. Note: RT-qPCR, reverse transcription quantitative polymerase chain reaction; AFAP1-AS1, actin filament-associated protein 1 antisense RNA 1; miR-384, microRNA-384; ACVR1, activin receptor A type I; Oct4, organic cation/carnitine transporter 4; CK19, keratin 19; ABCG2, breast cancer resistance protein; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; F, forward; R, reverse