Table 1 Primer sequences for RT-qPCR
Gene | Sequences |
|---|---|
AFAP1-AS1 | F: 5′-ACTGAAGAGGAACCAGGGACAG-3′ |
|  | R: 5′-GGGGAAACTGAAATGAATGAAG-3′ |
miR-384 | F: 5′-TGTTAAATCAGGAATTTTAA-3′ |
|  | R: 5′-TGTTACAGGCATTATGA-3′ |
ACVR1 | F: 5′-GTGAAGGTCTCTCCTGCGGTA − 3′ |
|  | R: 5′-GCCATCGTTGATGCTCAGTGA − 3′ |
Oct4 | F: 5’-CAAAGCAGAAACCCTCGTGC-3’ |
|  | R: 5’-AACCACACTCGGACCACTCG-3’ |
Nestin | F: 5’-ATCCCGTCAGCTGGAAAAGG-3’ |
|  | R: 5’-GGTGAGCTTGGGCACAAAAG-3’ |
CK19 | F: 5’-ACCATTGAGAACGCCAGGATT-3’ |
|  | R: 5’-TCCAGCACCCAAACACTCAA-3’ |
CD133 | F: 5′-TATAAAGCTTACCATGGCCCTCGTACTCGGCTC -3′ |
|  | R:5′-TATAGGATCCTCAATGTTGTGATGGGCTTGTC-3′ |
ABCG2 | F: 5′-CAGGTGGAGGCAAATCTTCGT-3′ |
|  | R: 5′-CTTGTACTCCGTCAGCGTGA-3′ |
GAPDH | F: 5’-ACAGTCCATGCCATCACTG-3’ |
|  | R: 5’-AGTAGAGGCAGGGATGATG-3’ |
U6 | F: 5’-TGCGGGTGCTCGCTTCGGCAGC-3′ |
|  | R: 5′-CCAGTGCAGGGTCCGAGGT-3′ |