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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Phosphoglycerate dehydrogenase promotes pancreatic cancer development by interacting with eIF4A1 and eIF4E

Fig. 2

Knockdown of PHGDH attenuates pancreatic cancer development through inhibiting cell proliferation and tumorigenesis. a Nude mice were subcutaneously inoculated with the constructed PANC-1 cells. Tumor sizes were measured every 3 or 4 days. Each value is the mean ± SEM of determinations in at least 6 nude mice of each group. b, c and d Nude mice were orthotopically inoculated with the constructed PANC-1 cells. b and c Liver and lung metastases (arrows, metastatic foci) were detected with HE staining. Scale bars, 200 μm. The quantitative results represented the relative area of metastatic foci to the whole tissue slide; d The survival statuses were analyzed for 8 weeks. Each group at least contained 6 nude mice. e and f Kaplan-Meier curve for overall survival of the pancreatic adenocarcinoma patients based on (e) PHGDH mRNA and (f) PHGDH and SHMT1 mRNAs expression in the TCGA dataset. The blue line represented the patients with (e) low PHGDH and (f) low PHGDH and low SHMT1 expression levels (z-score < − 0.5); the black line represented other patients (z-score ≥ − 0.5). e n = 178. f n = 80. g Relative proliferation results of PANC-1 cells under the treatments of 30 μM PHGDH inhibitor CBR5884, 50 μM cisplatin and/or 25 μM paclitaxel for 24 h. h Relative proliferation results of the constructed PANC-1 cells for 24 and 48-h cultures. i and j Relative proliferation results of the constructed PANC-1 cells under the treatments of 10 mM serine and/or 10 mM glycine. k and l Colony formation results of the constructed PANC-1 cells under (k) the normal conditions and (l) the conditions of serine and glycine free (up), 1 mM serine supply (middle) and 1 mM glycine supply (down) respectively. Data are representative of at least three independent experiments. p value: Student’s t-test; *p < 0.05,**p < 0.01, ***p < 0.001. Columns: mean; bars: SD

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