Fig. 3

PHGDH regulates cell-cell tight junction-related proteins expression. a Enlarged single colony images of the constructed PANC-1 cells in the colony formation assays. Scale bar, 100 μm. b Western blot and (c) Immunofluorescence results of the tight junction-related proteins in the constructed PANC-1 cells. Scale bar, 20 μm. d Western blot analysis of the indicated protein expression levels in PANC-1-shPHGDH cells under the treatment of 0, 1, 2, 10, 20 mM serine. e Western blot analysis of the indicated protein levels in the PANC-1 cells treated by CBR5884 for 12 h. f Relative total and labeled serine contents in the constructed AsPC-1 cells. Data are representative of at least three independent experiments. p value: Student’s t-test; **p < 0.01, ***p < 0.001. Columns: mean; bars: SD. g Western blot analysis of the indicated protein levels in the constructed AsPC-1 cells. h Immunofluorescence results of the indicated proteins in the constructed AsPC-1 cells. Scale bar, 20 μm. i Enlarged single colony images of the constructed AsPC-1 cells in the colony formation assays. Scale bar, 100 μm. j Western blot analysis of the indicated proteins in the PANC-1 cells treated by serine free condition