Fig. 4

PHGDH interacts with eIF4A1 and eIF4E in PANC-1 cells. a Immunoprecipitation results of the interaction between PHGDH (bait) and eIF4A1, in PANC-1-GFP and PANC-1-shPHGDH cells (left), AsPC-1-RFP and AsPC-1-PHGDH cells (right). b Reverse immunoprecipitation results of the interaction between PHGDH and eIF4A1 (bait). c Pull down assay results of the interactions between recombinant PHGDH (bait) protein, recombinant eIF4A1 protein, recombinant eIF4E protein and recombinant eIFG1 protein. “r” shorted for recombinant. d Immunofluorescence results of the overlapping signals between PHGDH and eIF4A1 or eIF4E in PANC-1-GFP and PANC-1-shPHGDH cells. Scale bar, 20 μm. e Immunofluorescence results of the overlapping signals between PHGDH and eIF4A1 or eIF4E in AsPC-1-RFP and AsPC-1-PHGDH cells. Scale bar, 20 μm