Fig. 6

Overexpression of PHGDH enhances translation initiation to further promote the pancreatic cancer development. a Western blot analysis of the phosphorylation of translation initiation related factors eIF4E, eIF4G1, eIF2α and 4E-BP1 in the constructed AsPC-1 cells. b and c Co-immnoprecipitation results of the integrity of translation initiation complex eIF4F in AsPC-1-RFP and AsPC-1-PHGDH cells under the treatment of 4EGI-1 for 12 h. d Polysomes profiling of AsPC-1-RFP and AsPC-1-PHGDH cells treated by 4EGI-1 for 12 h. e Extracted the polysomes associated mRNA of AsPC-1-RFP cells and AsPC-1-PHGDH cells treated by 4EGI-1 for 12 h and detected the ZO-1 and E-cadherin mRNA levels using qRT-PCR method. f Western blot analysis of the indicated protein levels under the treatment of 4EGI-1 in AsPC-1-RFP and AsPC-1-PHGDH cells. g Colony formation results of the constructed AsPC-1 cells under the normal conditions. h Results of animal experiments. The constructed AsPC-1 cells were inoculated subcutaneously into the nude mice. Tumor sizes were measured every 3 or 4 days. Each value is the mean ± SEM of determinations in at least 6 nude mice of each group. i Results of animal experiments. The constructed AsPC-1 cells were inoculated orthotopically into the nude mice. The survival statuses were analyzed for 8 weeks. Each group at least contained 6 nude mice. Data are representative of at least three independent experiments. p value: Student’s t-test; *p < 0.05,**p < 0.01. Columns: mean; bars: SD