Fig. 2

IR promoted cell motility and EMT in the presence of mut-p53 in vitro. a A wound-healing assay was conducted to test the migration ability of cells. Wounds were set into a confluent cell layer of three p53 status cell lines under IR or not. Photographs at time 0 and after 24 h were shown (left panel). Red bar, 100 μm. The distances of wound width were shown at the right panel. b The role of p53 status in IR-induced invasion was demonstrated by transwell assay in H1299 cells (left panel). Red bar, 100 μm. The number of migrated cells were shown (right panel). c Cells were stained with phalloidin to examine F-actin stress fibers. Images were captured on a Zeiss fluorescence microscope. Red bar, 10 μm. d Effects of IR on E-cadherin and N-cadherin expressions in three p53 status cell lines by western blotting analysis. e Immunofluorescence assay to determine the localization of EMT-associated proteins. Confocal microscopy images of E-cadherin, N-cadherin, Vimentin, and DAPI as “separate” or “merged” images. Red bar, 10 μm. f PCR assay analyzed the mRNA level of EMT-associated markers, Zeb1, Snail and twist1 in three p53 status cell lines under IR or not. Results shown are the representative of three identical experiments. *P < 0.05, **P < 0.01, *** P < 0.001, Student’s t-test